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• EnzFitter是一款通用的曲線擬合軟件，特別適用于分析酶動(dòng)力學(xué)實(shí)驗(yàn)。內(nèi)置模塊包括有或沒(méi)有底物抑制/非競(jìng)爭(zhēng)以及混合抑制的米式方程等模塊，并可以自定義添加模塊，非常適用于酶動(dòng)力學(xué)實(shí)驗(yàn)分析，例如：通過(guò)對(duì)單個(gè)或兩個(gè)基層數(shù)率的置信界限得到開(kāi)始的比率和參數(shù)值。
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• EnzFitter內(nèi)置強(qiáng)大的功能，可以批處理模式運(yùn)行，并且支持OLE自動(dòng)化。可以根據(jù)擬合的標(biāo)準(zhǔn)曲線計(jì)算未知數(shù)。可以創(chuàng)建附加到任何文件，數(shù)據(jù)或曲線擬合的文本注釋。
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• Biosoft EnzFitter主要特性：
• 1. EnzFitter是一種通用的曲線擬合軟件包，具有專門設(shè)計(jì)用于特別適用于酶動(dòng)力學(xué)實(shí)驗(yàn)分子的自定義功能。例如，初始速率和參數(shù)值可以通過(guò)他們對(duì)于單基質(zhì)速率數(shù)據(jù)和雙基質(zhì)速率數(shù)據(jù)的置信限來(lái)獲得。內(nèi)置模型包括具有或不具有底物抑制作用的Michaelis-Menten，競(jìng)爭(zhēng)性，無(wú)競(jìng)爭(zhēng)性和混合抑制作用，三元復(fù)合物或有序bi-bi系統(tǒng)以及有和無(wú)底物抑制的乒乓球。您可以使用傳統(tǒng)的代數(shù)語(yǔ)法輕松添加其他模型。
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• 2. 數(shù)據(jù)導(dǎo)入格式包括EnzFitter for DOS文件以及ASCII分隔符，dBase，F(xiàn)oxPro，Excel，Quattro Pro，Lotus123，Paradox和Symphony。文件和數(shù)據(jù)集大小僅受可用內(nèi)存的限制，并且可以為每個(gè)文件創(chuàng)建多個(gè)數(shù)據(jù)集。有先進(jìn)的數(shù)據(jù)編輯功能，包括：插入/編輯/刪除點(diǎn)，復(fù)制和移動(dòng)數(shù)據(jù)塊和編輯列標(biāo)題。照顧缺少數(shù)據(jù)和復(fù)制品。可以顯示和導(dǎo)出數(shù)據(jù)的描述性統(tǒng)計(jì)工具。工作表使可自定義的（例如字體，顏色，邊框，行高和列寬），當(dāng)打印數(shù)據(jù)時(shí)，可以給自定義頁(yè)眉或頁(yè)腳。您可以隨時(shí)插入新的列或行。提供了許多數(shù)據(jù)轉(zhuǎn)換，或者您可以創(chuàng)建自己的轉(zhuǎn)換方程。您可以為經(jīng)常使用的數(shù)據(jù)格式創(chuàng)建模板，并結(jié)合圖模板實(shí)現(xiàn)常規(guī)檢測(cè)的自動(dòng)化。
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• 3. 可以使用Marquardt或Simplex算法對(duì)任何數(shù)據(jù)集執(zhí)行多重曲線擬合。可以對(duì)數(shù)據(jù)進(jìn)行穩(wěn)健，統(tǒng)計(jì)，比例或顯式值加權(quán)。遺傳算法可以生成出色的初始參數(shù)估計(jì)值，但參數(shù)可以被限制在一定范圍內(nèi)，或者在必要時(shí)固定。結(jié)果以表格和圖形形式呈現(xiàn)，并可以繪制原始數(shù)據(jù)和擬合數(shù)據(jù)的推行，并且為了區(qū)分?jǐn)?shù)據(jù)集，您可以選擇各種符號(hào)，半連續(xù)線和添加標(biāo)簽。可以在主圖上插入額外數(shù)據(jù)集的附屬圖，相同數(shù)據(jù)的殘差和變換/衍生圖。
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• 4. EnzFitter可以設(shè)置為以批處理模式運(yùn)行（自動(dòng)執(zhí)行多個(gè)分析，無(wú)需用戶干預(yù)），并且還支持OLE自動(dòng)化。對(duì)于更一般的化驗(yàn)用途，您可以根據(jù)擬合的標(biāo)準(zhǔn)曲線計(jì)算未知數(shù)。您可以創(chuàng)建附加到任何文件，數(shù)據(jù)集或曲線擬合的文本注釋。還有一個(gè)自動(dòng)記錄日志記錄對(duì)給定文件所做的所有更改。
• Enzyme Kinetics and Curve Fitting
• EnzFitter is a generic curve-fitting package which has custom features designed to make it especially suitable for analysis of enzyme kinetics experiments. For example, initial rate and parameter values can be obtained with their confidence limits for single and twin substrate rate data. Built-in models include Michaelis-Menten with or without substrate inhibition, competitive, uncompetitive and mixed inhibition, ternary complex or ordered bi-bi systems and ping-pong with and without inhibition by substrates. You can easily add other models in conventional algebraic syntax.
• Data import formats include EnzFitter for DOS files as well as ASCII delimited, dBase, FoxPro, Excel, Quattro Pro, Lotus 123, Paradox and Symphony. File and dataset sizes are limited only by available memory and multiple data sets can be created for each file. There are advanced data editing facilities including: insert/edit/remove points, copy and move blocks of data and edit column titles. Missing data and replicates are catered for. Descriptive statistics of the data can be displayed and exported. The worksheet is customizable (e.g. font, colors, borders, row height and column width) and when data are printed they can be given custom headers or footers. You can insert new columns or rows at any time. Many data transforms are supplied or you can create your own equations for transforms. You can create templates for data formats you use frequently which, combined with graph templates virtually automate routine assays.
  
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• Multiple curve fits can be performed for any data set using either the Marquardt or Simplex algorithms. Optionally, data can be weighted robustly, statistically, proportionally or with explicit values. A genetic algorithm generates excellent initial parameter estimates but parameters can be constrained to a range of values or fixed when necessary. The results are presented in tabular and graphic form and can be saved to disk, printed or exported to other programs. Export formats include, for graphs, Bitmaps, Metafiles, JPG or TIF and, for numeric results, ASCII delimited and Excel. Graphs of raw and fitted data can be plotted and, to distinguish datasets, you can select a variety of symbols, semi-continuous lines and also add labels. Subsidiary graphs of extra sets of data, residuals and transformed /derivative plots of the same data can be inset on the main graph.
• EnzFitter can be set up to run in batch mode (performing several analyses automatically, without user intervention) and it also supports OLE Automation. For more general assay use, you can calculate unknowns from fitted standard curves. You can create text Notes that are attached to any file, dataset or curve fit. There is also an automatic Logbook which records all changes made to a given file.